Methodological background
of Cell Cycle Profiling

Starting with a small tumour biopsy (≥27mm³) a lysate is prepared in the homogeniser compartment of the cell cycle profiling instrument . The lysate is then split into two: one part of the lysate is transferred to a special protein chip device, the ImmobiChip, for measurement of the protein expression by use of fluorescently labelled antibodies .
The second part of the lysate is applied to antibody-loaded microcolumns . CDKs bound to the affinity resin are washed and incubated with an enzyme-specific substrate. By phosphorylating the substrate with fluorescently marked phosphate groups and application of this labelled substrate to the ImmobiChip the activity values of the CDKs are detected .
An easy to use software solution determines specific activity from the expression and activity levels and normalises these values using background correction, controls and standards.
By use of a specific algorithm the clinician will then be provided with a result from the software for the classification of the tumour as being a high-risk, intermediate-risk or low-risk concerning the relapse potential of the tumour, related to an expected tumour response or non-response to a certain chemotherapeutic drug .

WORKFLOW OF CELL CYCLE PROFILING